Cultural Characteristics of Streptococcus

4. Growth is poor on solid media or broth.
5. Grow well in media containing blood and sugar ( fermentable carbohydrates) and 10% CO2 in environment promotes growth and hemolysis.
6. Selective media containing aminoglycosides or 1:500,000 crystal violet selectively permit the growth of Streptococci in a mixed culture and inhibit other bacteria.
7. Colony on blood agar is non-pigmented.
8. Complete hemolysis ( B-hemolysis) on blood containing agar.
9. Alpha hemolysis shows greenish coloration due to hydrogen peroxide released from the colonies and inducing oxidation of hemoglobin to methemoglobin.
Beta-hemolysis on blood agar (Streptococcus pyogenes)
                             Beta-hemolysis on blood agar (Streptococcus pyogenes)


Alpha-hemolysis on blood agar (Streptococcus pneumoniae)
                       Alpha-hemolysis on blood agar (Streptococcus pneumoniae)

Cultural Characteristics of Streptococcus

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